The Capturem Streptavidin 96-Well Plate is a single-use, disposable plate for simple, rapid enrichment of target proteins and antibodies that bind biotinylated protein. The plate binds more than 15 µg of biotinylated BSA control per well. This plate is suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates (e.g., mammalian or bacterial cell lysates), and cell culture supernatants of hybridoma cell lines.
Capturem Streptavidin miniprep is a single-use disposable column for simple, rapid enrichment of target proteins and antibodies that bind biotinylated proteins. The membranes are guaranteed to bind more than 15 μg of biotinylated BSA control per column. These miniprep columns are suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates (e.g., mammalian or bacterial cell lysates), and cell culture supernatants of hybridoma cell lines.
Overview
- Simple, ultra-fast workflow: 5–15-minute room-temperature protocols
- High concentration: extremely small elution volumes yield highly concentrated complexes
- High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
- High reproducibility: consistent results from well to well
- Single use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover
More Information
Applications
- Purification or enrichment of biotinylated proteins
- Pull-down of binding partners (e.g., proteins or oligos) for a biotin-labeled ligand
Loading capacities and times for available Capturem Streptavidin formats
| |
Biotin-antibody |
Biotin-BSA |
Biotin-ssDNA |
Free biotin |
Purification time |
| Capturem Streptavidin miniprep columns and 96-well plates |
20–40 µg |
>15 µg |
1,000–1,500 ng |
>4,000 pmol |
15 minutes |
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