Rapid, Protein A-based IP—Capturem IP & Co-IP Kit

CLT635721

Immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) are valuable tools for studying the interaction of proteins with macromolecules, such as other proteins. The Capturem IP & Co-IP Kit provides a complete, easy-to-use solution for the rapid isolation of individual proteins or protein-protein complexes. The kit features Capturem Protein A columns for high-capacity, membrane-based purification of antibody-bound protein complexes. All of the buffers necessary for cell lysis as well as binding, washing, and eluting of the antibody-antigen complexes are also included in the kit. IPs are performed in just 15 minutes, from sample incubation with antibody through elution.

Brand: Takara

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CLT635721 Capturem™ IP & Co-IP Kit, 12 Purifications	Capturem™ IP & Co-IP Kit, 12 Purifications	Login for price

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The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis.

Overview

Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
High concentration: extremely small elution volumes yield highly concentrated complexes
High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
Versatility: compatible with a wide range of IP buffers and conditions
Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

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Applications

Immunoprecipitation
Co-immunoprecipitation
Investigation of protein complexes
Antigen screening for antibody discovery and engineering workflows

Isotype affinity of Protein A and Protein G

Species	Antibody subtype	Protein A	Protein G	Protein A/G
Human	Total IgG IgG1 IgG2 IgG3 IgG4 IgM IgD IgA1 IgA2 Fab scFv	+++ +++ +++ + +++ + − + + + +	+++ +++ +++ +++ +++ − − − − + −	+++ +++ +++ +++ +++ + − + + + +
Mouse	Total IgG IgM IgG1 IgG2a IgG2b IgG3	+++ − + +++ +++ +++	+++ − ++ +++ +++ +++	+++ − ++ +++ +++ +++
Rat	Total IgG IgG1 IgG2a IgG2b IgG2c	+ + − − +++	++ ++ +++ + +++	++ ++ +++ + +++
Rabbit	Total IgG	+++	+++	+++

Key: +++ strong binding; ++ medium binding; + weak binding; − no binding

Protein Purification

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