Capturem His-tagged 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of his-tagged proteins. These plates combine Ni-NTA chemistry with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for his-tagged proteins are up to 800 µg per well with a 4.5-ml sample capacity per well.
Capturem His-Tagged Purification 96 is a single-use, disposable 96-well plate for simple, rapid purification of his-tagged proteins from up to 1 ml of clarified lysate per well. This plate is suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Capturem His-Tagged Purification 96 is compatible with automated liquid handlers equipped with vacuum filtration or centrifuges equipped with appropriate plate holders.
Capturem His-Tagged Purification Large Volume lets you quickly purify up to 25 mg of protein (depending on protein size and expression level) from a large volume of lysate (up to 500 ml at a time), resulting in purified protein concentrated at up to 2 mg/ml. Like our other his-tagged Capturem products, the Large Volume unit works for bacterial and mammalian expression systems with a wide range of additives.
Capturem His-Tagged Purification Maxiprep Columns are single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 25 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol.
The Capturem His-Tagged Purification Maxiprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 25 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
More Information
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
Approximate yields for Capturem his-tagged formats
| Format |
Yield* |
Purification time |
| Miniprep |
80 µg |
5 min |
| 96-well plate |
80 µg |
15 min |
| 24-well plate |
800 µg |
15 min |
| Maxiprep |
1.5 mg |
15 min |
| Large volume |
10–25 mg |
30 min |
*Yields vary depending upon the sample type, species, and antibody isotype.
Menu
X
