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  6. PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) - TKRR047

PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) - TKRR047

Genomic DNA contamination can be a significant problem during real-time RT-PCR because co-amplification of genomic DNA and cDNA can lead to erroneous results. With the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time), genomic DNA contamination is eliminated. This reverse transcription kit enables fast cDNA synthesis for real-time RT-PCR (qPCR) and includes a rapid step that eliminates genomic DNA (gDNA) contamination without loss of input RNA.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TKRR047B PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time), 400 Rxns PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time), 400 Rxns Login for price  
TKRR047A PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time), 100 Rxns PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time), 100 Rxns Login for price  

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PrimeScript RT reagent Kit with gDNA Eraser is a reverse-transcription kit for real-time RT-PCR (RT qPCR) that includes a genomic DNA elimination reaction. cDNA synthesis from RNA can be achieved without sample loss in a rapid reaction that is complete in less than 20 minutes. Genomic DNA is eliminated by treatment for 2 minutes at 42°C with gDNA Eraser, which has potent DNA degradation activity. Then a reverse-transcription reaction reagent is added that includes a component which completely inhibits DNA degradation activity, and the reverse-transcription reaction proceeds for 15 minutes. The cDNA obtained using this product can be used with either an intercalating green dye qPCR assay or probe qPCR assay. Please use in combination with quantitative PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A) and Probe qPCR Mix (Cat. #RR391A)

Overview

  • Genomic DNA contamination elimination in only 2 minutes
  • Fast cDNA synthesis—reverse transcription in just 15 minutes
  • Suitable for use with routine and challenging RNA templates alike
  • Full-length cDNA synthesis up to 12 kb

Applications

  • Elimination of genomic DNA contamination
  • cDNA synthesis for real-time RT-PCR (qPCR)
  • Two-step real-time RT-PCR