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Real-time RT-PCR Kit: PrimeScript RT Master Mix (Perfect Real Time)

To minimize sample handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase Inhibitor, random 6mers, Oligo dT Primer, dNTP Mixture, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TKRR036A PrimeScript™ RT Master Mix (Perfect Real Time), 200 Rxns PrimeScript™ RT Master Mix (Perfect Real Time), 200 Rxns Login for price  
TKRR036B PrimeScript™ RT Master Mix (Perfect Real Time), 800 Rxns PrimeScript™ RT Master Mix (Perfect Real Time), 800 Rxns Login for price  

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Features

  • Reverse transcription in just 15 minutes
  • Master Mix format reduces pipetting steps and the possibility of contamination
  • Works efficiently with GC-rich and/or higher-structured RNA templates
  • Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis

This real-time RT-PCR kit includes PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity for optimal efficiency during cDNA synthesis. The PrimeScript recombinant RTase enzyme is robust, versatile and well-suited for applications requiring full-length cDNA synthesis. PrimeScript RTase enables reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure, making this real-time RT-PCR kit ideal for both routine applications and challenging reverse transcriptase reactions alike. PrimeScript RTase is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) enzyme and is verified to be RNase H Minus. PrimeScript Reverse Transcriptase is engineered to allow full-length cDNA synthesis reactions at lower temperatures (e.g., 42°C), decreasing the risk of RNA degradation that occurs during conventional reactions performed at higher temperatures.