The PrimeScript One Step RT-PCR Kit Ver.2 is designed for RT-PCR in a single tube. All components necessary for RT-PCR are mixed in one tube, and therefore it is unnecessary to add reagents at the midpoint of the procedure and the risk of contamination is minimized. This kit includes PrimeScript 1 step Enzyme Mix (a highly optimized premix of PrimeScript Reverse Transcriptase, which has excellent extension even for RNA templates containing higher-order structure, the high efficiency hot-start PCR enzyme Takara Ex Taq HS, and RNase Inhibitor together with a stabilizing agent) and 2X 1 step buffer (a premix containing reaction buffer, dNTP mixture, and 1 step Enhancer Solution). These premix components allow easy preparation of reaction mixtures. This kit facilitates efficient production of RT-PCR products through efficient reverse transcription at 50°C by permitting reaction conditions and reduction in non-specific amplification that can arise from mispriming or from primer-dimer during pre-cycling steps. This kit includes all reagents necessary for reverse transcription of RNA to cDNA and cDNA amplification using PCR. Cat. # RR055B contains 4 of Cat. # RR055A. Please refer to Cat. # RR055A for complete product documentation and resources.
The PrimeScript One Step RT-PCR Kit Ver.2 (Dye Plus) is used for one-step RT-PCR in a single tube. There is no need to add reagents during the reaction, minimizing the risk of contamination. Furthermore, electrophoresis loading dyes (blue and yellow) are included in the premix to allow direct analysis by electrophoresis. The bright green color of the reaction mixture allows visual confirmation of gel loading. The kit includes PrimeScript RTase, a reverse transcriptase that provides superior elongation, and Takara Ex Taq HS, a PCR enzyme formulated for hot start PCR that provides high yields. The reaction components are optimized to provide the same level of performance as conventional RT-PCR systems that lack dyes and loading buffer. This kit includes all of the reagents necessary for the synthesis of cDNA from RNA by reverse transcription and amplification of cDNA by PCR.
Features
- Efficient, fast RT-PCR in a single tube
- Works efficiently with GC-rich and highly structured RNA templates
- TaKaRa Ex Taq HS allows specific amplification with minimal background
- Quick and easy reaction preparation
- Choose from one-step RT-PCR kits with or without gel loading buffer included in the reaction
Applications
- One-step RT-PCR
- Gene expression analysis by endpoint RT-PCR
Components
- RR055A (50 reactions; 50 µl per reaction)
| PrimeScript One Step Enzyme Mix |
100 µl |
| 2X One Step Buffer |
2 × 625 µl |
| Control F-1 Primer* (20 µM) |
20 µl |
| Control R-1 Primer** (20 µM) |
20 µl |
| Positive Control RNA (2 x 105 copies/µl) |
20 µl |
| RNase Free dH2O |
2 × 625 µl |
* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA
- RR055B (200 reactions; 50 µl per reaction)
This kit is comprised of 4 x RR055A.
- RR057A (50 reactions; 50 µl per reaction)
| PrimeScript One Step Enzyme Mix |
100 µl |
| 2X One Step Buffer (Dye Plus) |
2 × 625 µl |
| Control F-1 Primer* (20 µM) |
20 µl |
| Control R-1 Primer** (20 µM) |
20 µl |
| Positive Control RNA (2 x 105 copies/µl) |
20 µl |
| RNase Free dH2O |
2 × 625 µl |
* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA
- RR057B (200 reactions; 50 µl per reaction)
This kit is comprised of 4 x RR057A
Storage
–20°C
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