The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR.
Overview
- Enables accurate and rapid analysis of viral RNA or RNA derived from rare or precious sources.
- Contains Takara Ex Taq HS, a high-efficiency, hot-start PCR enzyme, for PCR.
- Buffer system is real-time PCR optimized, increasing amplification efficiency and detection sensitivity.
Applications
- One-step real-time RT-PCR
- Probe-based qPCR assays such as 5' nuclease assays and real-time PCR (qPCR) assays