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One Step PrimeScript III RT-PCR Kit - TKRR600

One Step PrimeScript III RT-qPCR Mix is a 2X master mix for one-step real-time, probe-based RT-qPCR (using the 5′ nuclease method). It contains a novel heat- and inhibitor-resistant reverse transcriptase, PrimeScript III RTase, in combination with an efficient, specific hot-start PCR enzyme, TaKaRa Taq HS, and an optimized reaction buffer. A quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TKRR600A One Step PrimeScript™ III RT-PCR Kit, 200 Rxns One Step PrimeScript™ III RT-PCR Kit, 200 Rxns Login for price  
TKRR600S One Step PrimeScript™ III RT-PCR Kit, 50 Rxns One Step PrimeScript™ III RT-PCR Kit, 50 Rxns Login for price  
TKRR600B One Step PrimeScript™ III RT-PCR Kit, 1,000 Rxns One Step PrimeScript™ III RT-PCR Kit, 1,000 Rxns Login for price  

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Overview

  • Fast, easy one-step RT-qPCR in a single tube using probe-based detection
  • Efficient, specific cDNA synthesis and amplification, even from complex RNA templates
  • Suitable for a wide variety of samples from different sources, including crude RNA extracts

Applications

  • Gene expression
  • RNA virus detection
  • Multiplex real-time PCR

Instrument Compatibility

  • Thermal Cycler Dice Real Time System (TaKaRa Cat. #TP800)2
  • ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems)
  • LightCycler (Roche Diagnostics)
  • SmartCycler System/SmartCycler II System (Cepheid)