Protino Ni-NTA Columns

Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification

Select your options below

Product CodeProduct	Product Description	Price (Excl gst)
MN745415.1 Protino Ni-NTA Column 5 mL (1)	Protino Ni-NTA Column 5 mL (1)	Login for price
MN745415.5 Protino Ni-NTA Columns 5 mL (5)	Protino Ni-NTA Columns 5 mL (5)	Login for price
MN745410.5 Protino Ni-NTA Columns 1 mL (5)	Protino Ni-NTA Columns 1 mL (5)	Login for price

Features

Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
High capacity and high affinity
Simply replace your current Ni-NTA products, no optimization or protocol change necessary
Purification under native and denaturing conditions
Suitable for small proteins, large protein complexes, proteins with low expression rates - universal use
Market-leading performance for an unbeatable price
Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose

	Protino® Ni-NTA Columns 1 mL	Protino® Ni-NTA Columns 5 mL
Technology	IMAC (immobilized metal ion affinity chromatography)	IMAC (immobilized metal ion affinity chromatography)
Chelating ligand	NTA (nitrilotriacetic acid)	NTA (nitrilotriacetic acid)
Format	1 mL FPLC™ columns	5 mL FPLC™ columns
Column dimensions	0.7 cm (inner diameter) x 2.5 cm (height)	1.6 cm (inner diameter) x 2.5 cm (height)
Column body material	Polypropylen	Polypropylen
Column inlet port	10-32 (1/16'') female	10-32 (1/16'') female
Column outlet port	10-32 (1/16'') male	10-32 (1/16'') male
Matrix	6 % beaded agarose (cross-linked), precharged with Ni2+	6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size	45–165 µm	45–165 µm
Binding capacity*	50 mg	250 mg
Recommended flow rate**	1 mL/min	5 mL/min
Max. recommended flow rate	4 mL/min	10 mL/min
Storage solution	30 vol % ethanol	30 vol % ethanol
Storage temperature	4–8 °C	4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein ** High flow rates may reduce binding capacity

Protein Kits