Protino Ni-NTA Agarose

Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification

Select your options below

Product CodeProduct	Product Description	Price (Excl gst)
MN745400.100 Protino Ni-NTA Agarose (100 mL)	Protino Ni-NTA Agarose (100 mL)	Login for price
MN745400.25 Protino Ni-NTA Agarose (25 mL)	Protino Ni-NTA Agarose (25 mL)	Login for price
MN745400.500 Protino Ni-NTA Agarose (500 mL)	Protino Ni-NTA Agarose (500 mL)	Login for price

Features

Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
High capacity and high affinity
Simply replace your current Ni-NTA products, no optimization or protocol change necessary
Purification under native and denaturing conditions
Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use
Market-leading performance for an unbeatable price
Aqueous suspension
For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
Empty Protino® Columns for gravity flow available

Technology	IMAC (immobilized metal ion affinity chromatography)
Chelating ligand	NTA (nitrilotriacetic acid)
Format	50 % (v/v) aqueous suspension containing 30 % ethanol
Matrix	6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size	45–165 µm
Binding capacity*	Up to 50 mg/mL settled agarose
Storage temperature	4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein

Nucleic Acid Extraction