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Protino Ni-IDA Resin

Protino® Ni-TED, the pure alternative for His-tag protein purification

Brand: Macherey-Nagel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MN745210.120 Protino Ni-IDA Resin (120 g) Protino Ni-IDA Resin (120 g) Login for price  
MN745210.30 Protino Ni-IDA Resin (30 g) Protino Ni-IDA Resin (30 g) Login for price  
MN745210.5 Protino Ni-IDA Resin (5 g) Protino Ni-IDA Resin (5 g) Login for price  
MN745210.600 Protino Ni-IDA Resin (600 g) Protino Ni-IDA Resin (600 g) Login for price  

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Features

  • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies)
  • High stability against reducing / chelating agents
  • Low metal leaching
  • High protein purity
  • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
  • Dry resin – storage at room temperature
  • Dry bulk material
  • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility
  • Batch procedure, gravity-flow column chromatography, FPLC™ applications
  • Empty Protino® columns available for use with Protino® Ni-TED Resin
  • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin

Product

Preps

Specification

REF

Protino® Ni-TED Resin

5 g

dry silica bulk resin

MN745200.5

 

30 g

as above

MN745200.30

 

120 g

as above

MN745200.120

 

600 g

as above

MN745200.600

Technology

IMAC (immobilized metal ion affinity chromatography)

Chelating ligand

TED (tris-carboxymethyl ethylene diamine)

Format

Bulk matrix

Matrix

Macroporous silica

Physical form

Dry matrix, precharged with Ni2+

Binding capacity*

10 mg/g resin
5 mg/mL bed volume

Max. pressure

145 psi (10 bar)

* Binding capacity will vary for each polyhistidin-tagged protein