Next Gen Sequencing Kits 

• Easy, plate-based workflow—direct input of single cells isolated by FACS or other methods
• Unparalleled sensitivity and reproducibility—best performance for single-cell RNA-seq (especially for cells with very low RNA content and for nuclei), with the highest gene detection
• Robust, full-length chemistry—validated kit outperforms all existing single-cell full-length methods, including Smart-seq2
• Highly scalable workflow—high-throughput capabilities and automation-friendly

Applications

• cDNA synthesis from single cells for full-length transcriptome sequencing
• cDNA outputs from this kit can be used with Nextera® XT library preparation kits for Illumina sequencing

SMART-Seq Single Cell Kit provides higher sensitivity than Smart-seq2

Validated kit backed by expert technical support

The SMART-Seq Single Cell Kit outperforms Smart-seq2. Single cells from the lymphoblastoid cell line GM12878 were processed with the SMART-Seq Single Cell Kit (SSsc; 18 cells) or the Smart-seq2 method (Smart-seq2; 20 cells) using 19 cycles of PCR. RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.75 million paired-end reads). Panel A. The read distribution was different between the two chemistries, with a drastically higher number of reads mapping to the mitochondrial genome (and therefore, fewer reads available for gene identification) with Smart-seq2 chemistry. Panel B. More genes were detected in the cells processed with SSsc. For additional data on reproducibility and sensitivity, view our technical note.

Even higher sensitivity for single-cell applications

We have further modified our core technology to create a new chemistry with higher sensitivity designed specifically for single-cell applications. We demonstrated that the new SSsc kit generates data with even better sensitivity and reproducibility than the SMART-Seq v4 kit (SSv4).

The SMART-Seq Single Cell Kit's improved chemistry is highly suitable for single-cell applications. Panel A. 12 single cells from lymphoblastoid cell line GM22601 were processed with SSv4 or SSsc using 19 cycles of PCR. All samples were normalized to 1.25 million paired-end reads. The cDNA yield generated with SSsc was drastically higher than that generated with SSv4. Panel B. About 50 single PBMCs from one donor were processed with SSv4 or SSsc. About 60% more genes were detected in the cells processed with SSsc, regardless of the number of reads used for the analysis.