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  7. Protino Glutathione Agarose 4B

Protino Glutathione Agarose 4B

Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification

Brand: Macherey-Nagel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MN745500.10 Protino Glutathione Agarose 4B (10 mL) Protino Glutathione Agarose 4B (10 mL) Login for price  
MN745500.100 Protino Glutathione Agarose 4B (100 mL) Protino Glutathione Agarose 4B (100 mL) Login for price  

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Features

  • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
  • Simply replace your current products, no optimization or protocol change necessary
  • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use
  • Market-leading performance for an unbeatable price
  • Aqueous suspension
  • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications
  • Empty Protino® columns for gravity flow available

Technology

Affinity chromatography

Chelating ligand

Glutathione, linked via sulfur atom

Format

75 % (v/v) aqueous suspension containing 20 % ethanol

Matrix

4 % beaded agarose

Bead size

90 µm

Binding capacity*

8 mg/mL

Recommended flow rates

1 mL bed volume (column with 6.6 mm inner diameter):

Sample loading**:

0.2–1.0 mL/min

Washing / elution:

1.0 mL/min
 

10 mL bed volume (column with 16 mm inner diameter):

Sample loading**:

0.5–5.0 mL/min

Washing / elution:

5.0 mL/min

Max. linear flow rate

250 cm/h

Chemical stability

Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4, 
0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature

4–8 °C

Note

Do not autoclave the gel

*   Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein