This cDNA synthesis kit is powered by PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity and efficiently synthesizes cDNA. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques including RT-PCR, cDNA probe preparation and real-time quantitative RT-PCR (qRT-PCR or RT-qPCR). The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.
Features
- This cDNA synthesis kit includes oligo-dT and random 6-mer primers
- Strong strand displacement and extension capability: PrimeScript RTase is capable of synthesizing long and full-length cDNA molecules (up to 12 kb)
- High specificity: synthesize cDNA at 42°C - low background, high yield, and high quality RNA at a temperature that does not promote RNA degradation
- Outstanding accuracy: Lowest error rate among five commercially available reverse transcriptases tested
- Easy to use: Low rates of non-specific annealing, even on incompletely denatured RNA
- Works on challenging templates: Excellent results even with templates that are GC-rich or have high levels of secondary structure
- Highly sensitive: use less of your precious RNA samples
- Demonstrated success with RT reaction times of 30 min. and 60 min (for longer transcripts).
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