PrimeScript RT-PCR Kit

The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RT, an RNase H Minus MMLV reverse transcriptase enzyme that provides robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RT, and Takara Ex Taq HS polymerase. PrimeScript RT enables full-length cDNA synthesis from GC-rich and even highly structured RNA templates, while Takara Ex Taq HS allows for efficient, sensitive, and specific cDNA amplification. Reverse transcription can be performed at 42°C, avoiding the risk of RNA degradation, which may occur at higher temperatures. The PrimeScript RT-PCR Kit generates high-yield, full-length cDNA up to 12 kb in length.

Brand: Takara

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
TKRR014B PrimeScript™ RT-PCR Kit, 200 Rxns	PrimeScript™ RT-PCR Kit, 200 Rxns	Login for price
TKRR014A PrimeScript™ RT-PCR Kit, 50 Rxns	PrimeScript™ RT-PCR Kit, 50 Rxns	Login for price

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This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq HS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR.

Overview

RT-PCR kit includes all reagents needed for two-step endpoint RT-PCR (e.g., where products are analyzed using gel electrophoresis)
Robust reverse transcription and amplification even from challenging RNA templates
Complete elongation of complex RNA templates
High-yield transcription of full-length cDNA up to 12 kb in length
Minimal false priming and robust amplification due to the combination of PrimeScript RT with Takara Ex Taq HS

Applications

Two-step RT-PCR

PCR Reagents

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