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  5. Nucleic Acid Extraction
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  7. rDNase Set - MN740963

rDNase Set - MN740963

MN740963

Highly specific recombinant DNase for efficient removal of contaminating DNA

Brand: Macherey-Nagel

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Features

  • On-column DNA digestion – for time saving DNA removal during RNA preparation
  • DNA digestion in solution allows most efficient DNA removal for sensitive applications
  • No RNase activity detectable
  • RNA integrity (RIN) is not affected by rDNase treatment

Technology

Recombinant enzyme

Source

Recombinantly produced in Pichia pastoris without using any animal cells or other material derived from animals

Format

Lyophilized enzyme, separate Reaction Buffer

RNase activity

Not detectable

RNA integrity

RNA integrity is unchanged by treatment with MACHEREY-NAGEL rDNAse under recommended conditions

On-column DNA-removal reactions

The amount of rDNase and Reaction Buffer is sufficient for:

Mini spin columns:
NucleoSpin® RNA: 50 preps
NucleoSpin® miRNA: 70 preps
NucleoSpin® miRNA Plasma: 140 preps
NucleoSpin® RNA/Protein: 200 preps
NucleoSpin® TriPrep: 200 preps
NucleoSpin® RNA Blood: 125 preps
NucleoSpin® RNA Plant: 50 preps
NucleoSpin® totalRNA FFPE: 625 preps
NucleoSpin® RNA Clean-up: 250 preps

Mini spin columns (XS design):
NucleoSpin® RNA XS: 800 preps
NucleoSpin® totalRNA FFPE XS: 1250 preps
NucleoSpin® RNA Clean-up XS: 800 preps

Midi spin columns:
NucleoSpin® RNA Midi: 100 preps
NucleoSpin® RNA Blood Midi: 50 preps

8-well format:
NucleoSpin® 8 RNA: 240 preps
NucleoSpin® 8 RNA Blood: 240 preps

96-well format:
NucleoSpin® 96 RNA: 240 preps
NucleoSpin® 96 RNA Blood: 240 preps

Digestion in solution

50 x 1 mL