NucleoSpin RNA

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NucleoSpin RNA is a spin column based kit for manual use. It is intended for the isolation of high purity and integrity RNA from a wide range of sample materials like tissues, cultured cells, yeast and bacteria. Besides spin columns and buffers it contains rDNAse for on column DNA digestion.

Brand: Macherey-Nagel

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Product CodeProduct	Product Description	Price (Excl gst)
MN740955.10 NucleoSpin RNA (10)	NucleoSpin RNA (10)	Login for price
MN740955.50 NucleoSpin RNA (50)	NucleoSpin RNA (50)	Login for price
MN740955.250 NucleoSpin RNA (250)	NucleoSpin RNA (250)	Login for price
MN740955.50S NucleoSpin RNA Columns (50)	NucleoSpin RNA Columns (50)	Login for price
MN740955.250S NucleoSpin RNA Columns (250)	NucleoSpin RNA Columns (250)	Login for price

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Features

rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
Up to 70 μg ready-to-use RNA
Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set

Product	Preps	Specification	REF
NucleoSpin® RNA	10	NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase	MN740955.10
	50	as above	MN740955.50
	250	as above	MN740955.250
	240	Kit for use on QIAcube®* NucleoSpin RNA® Columns with Collection Tubes, NucleoSpin® Filters, buffers, RNase-free rDNase	MN740955.240C

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 5 x 106 cultured cells, < 109 bacterial cells, < 108 yeast cells, < 30 mg human / animal tissue
Fragment size	> 200 nt
Typical yield	14 μg (106 HeLa cells), 70 μg (109 bacterial cells)
A260/A280	1.9–2.1
Typical RIN (RNA integrity number)	> 9
Elution volume	40–120 µL
Preparation time	35 min/6 preps
Binding capacity	200 µg