Guide-it™ Indel Identification Kit - CLT631444

CLT631444

The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) created using nuclease based genome editing technologies, including CRISPR/Cas9, TALENs, and zinc finger nucleases, in a cell population. The kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. The indel identification protocol in this kit has been optimized to provide quick and reliable results.

Brand: Takara

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CLT631444 Guide-it™ Indel Identification Kit	Guide-it™ Indel Identification Kit	Login for price

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Features

Streamlined method for characterizing the variety of indels introduced by gene editing technologies
Ultrafast protocol includes Terra PCR Direct Polymerase for PCR amplification directly from cells, and the In-Fusion cloning system for ligation-free cloning in 15 minutes
Ideal for identifying indels caused by Cas9/CRISPR, TALENs, or zinc-finger nucleases based gene editing in a cell population
Complete kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis

Components

Guide-it Indel Identification Components
- 110 µl Terra PCR Direct Polymerase Mix
- 3 x 1 ml 2X Terra PCR Direct Buffer (with Mg²⁺, dNTP)
- 400 µl Extraction Buffer 1
- 40 µl Extraction Buffer 2
- 10 µl pUC19 Cloning Vector, linearized (50 ng/µl)
- 200 µl Colony PCR Forward Primer (15 µM)
- 200 µl Colony PCR Reverse Primer (15 µM)
- 6 x 1 ml PCR-Grade Water

In-Fusion HD Cloning Kit
NucleoSpin Gel and PCR Clean-Up Kit
Stellar Competent Cells

Gene Function

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