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  6. Guide-it™ CRISPR/Cas9 System (Green or Red)

Guide-it™ CRISPR/Cas9 System (Green or Red)

The Guide-it CRISPR/Cas9 Systems are kits for the cloning and expression of target single guide RNAs (sgRNAs) for mammalian genome editing using CRISPR/Cas9 technology. The vector in this system simultaneously expresses Cas9 nuclease, a target-specific sgRNA, and an exceptionally bright fluorescent protein for monitoring transfection efficiency and/or for further enriching/isolating transfected cells by flow cytometry (ZsGreen1 and tdTomato versions are available).

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT632601 Guide-it™ CRISPR/Cas9 System (Green) Guide-it™ CRISPR/Cas9 System (Green) Login for price  
CLT632602 Guide-it™ CRISPR/Cas9 System (Red) Guide-it™ CRISPR/Cas9 System (Red) Login for price  

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The kit includes Stellar competent cells to ensure high efficiency transformation.

Features

  • A single vector expresses your target-specific sgRNA from the human U6 promoter, and Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter
  • tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and/or to enrich/isolate transfected cells by flow cytometry
  • Simple plasmid construction—design oligos specific to your target sequence, and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells
  • Includes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids
Components
Guide-it CRISPR/Cas9 System (Green) (Cat. No. 632601)
  • pGuide-it-ZsGreen1 Vector (Linear)
  • Guide-it Ligation Components
  • Stellar Competent Cells
Guide-it CRISPR/Cas9 System (Red) (Cat. No. 632602)
  • pGuide-it-tdTomato Vector (Linear)
  • Guide-it Ligation Components
  • Stellar Competent Cells