Guide-it™ Mutation Detection Kit

The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products.

Brand: Takara

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
CLT631443 Guide-it™ Mutation Detection Kit, 100 Rxns	Guide-it™ Mutation Detection Kit, 100 Rxns	Login for price
CLT631448 Guide-it™ Mutation Detection Kit, 25 Rxns	Guide-it™ Mutation Detection Kit, 25 Rxns	Login for price

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Features

Simple method to identify insertions or deletions in mammalian cells
Faster and more efficient than the surveyor assay; amplify directly from your target cells
Complete kit: Guide-it Resolvase, Terra PCR Direct Polymerase and all buffers included
Value: 100 reactions per kit; also available in a 25 reaction size for small-scale genome editing projects
Perfect for confirming sequence changes downstream from Cas9/CRISPR, TALENs or zinc-finger nuclease studies

Gene Function

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