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Parallel isolation of RNA and protein from undivided samples
Brand: Macherey-Nagel
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NucleoSpin miRNA
Product Code: MN740971.10
NucleoSpin Plasmid Transfection Grade
Product Code: MN740490.10
Eppendorf Centrifuge 5810, without rotor - EP5810000084
Product Code: EP5810000084
Features
Product
Preps
Specification
REF
NucleoSpin® RNA/Protein
10
NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
MN740933.10
50
as above
MN740933.50
250
MN740933.250
Technology
Silica-membrane technology
Format
Mini spin columns
Sample material
< 5 x 106 cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue
RNA
Protein
Fragment size
> 200 nt
15–300 kDa
Typical yield
< 70 µg
< 1200 µg
A260/A280
1.9–2.1
–
Typical RIN (RNA integrity number)
> 9
Elution volume (RNA)Resolubilization volume (protein)
40–120 µL
10–100 µL
Preparation time
30 min/6 preps
35 min/6 preps
Binding capacity
200 µg