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NucleoSpin RNA Plus

New generation for efficient and quick RNA isolation from cells and tissues

Brand: Macherey-Nagel

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
MN740984.10 NucleoSpin RNA Plus (10)	NucleoSpin RNA Plus (10)	Login for price
MN740984.50 NucleoSpin RNA Plus (50)	NucleoSpin RNA Plus (50)	Login for price
MN740984.250 NucleoSpin RNA Plus (250)	NucleoSpin RNA Plus (250)	Login for price

Features

NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
Convenient handling – lysate clearing and gDNA removal with one column in one step
New efficient lysis buffer – no b-mercapthoethanol or TCEP necessary

Product	Preps	Specification	REF
NucleoSpin® RNA Plus	10	NucleoSpin® RNA Plus Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers	MN740984.10
	50	as above	MN740984.50
	250	as above	MN740984.250

Technology	Two column silica-membrane system 1. column for DNA removal 2. column for RNA isolation
Format	Mini spin columns
Sample material	< 1 x 107 cultured cells, < 109 bacterial cells, < 108 yeast cells, < 30 mg human / animal tissue
Fragment size	> 200 nt
Typical yield	HeLa cells 5 x 106: 40-60 µg Mouse liver 20 mg: 80-100 µg Mouse kidney 20 mg: 40-70 µg Mouse spleen 5 mg: 30-60 µg
A260/A280	1.9–2.1
A260/A230	1.8-2.5
Typical RIN (RNA integrity number)	> 9
Elution volume	30–120 µL
Preparation time	20 min/6 preps
Binding capacity	200 µg

RNA purification with MN – NucleoSpin® RNA and NucleoSpin® RNA Plus

How to use the NucleoMag® SEP Mini