NucleoBond PC EF

MN740548

The classic anion exchanger for purification of endotoxin-free plasmid DNA

Select your options below

Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
MN740548 NucleoBond PC 10000 EF (5)	NucleoBond PC 10000 EF (5)	Login for price

Features

Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
Patented endotoxin removal by additional washing step – no extra ice incubation necessary
Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces
NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation
Proven anion-exchange technology – transfection-grade plasmid DNA
Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns

Product	Preps	Specification	REF
NucleoBond® PC 500 EF	10	NucleoBond® AX 500 Columns, buffers, RNase A, NucleoBond® Folded Filters XL	MN740550
NucleoBond® PC 2000 EF	5	NucleoBond® AX 2000 Columns, buffers, RNase A, NucleoBond® Bottle Top Filters	MN740549
NucleoBond® PC 10000 EF	5	NucleoBond® AX 10000 Columns, buffers, RNase A, NucleoBond® Bottle Top Filters	MN740548
NucleoBond® PC Prep 100	1	1 NucleoBond® AX Prep 100 Column, 3 NucleoBond® AX 100 Columns, buffers, RNase A, Sieving Fabric, NucleoBond®Folded Filters	MN740594

	NucleoBond® PC 500 EF	NucleoBond® PC 2000 EF	NucleoBond® PC 10000 EF	NucleoBond® PC Prep 100
Technology	Anion-exchange chromatography
Format	Maxi	Mega	Giga	Preparative Scale
	Gravity-flow columns	Gravity-flow columns	Gravity-flow columns	Compatible with e.g., HPLC, FPLC
Lysate clarification	Folded filters	Bottle top filters	Bottle top filters	Folded filters, sieving fabric
Sample material	30–150 mL	150–500 mL	500–2000 mL	5–20 L
Vector size	< 300 kbp	< 300 kbp	< 300 kbp	< 300 kbp
Typical yield	100–500 µg	500–2000 µg	2000–10000 µg	80–100 mg
A260/A280	1.80–1.95	1.80–1.95	1.80–1.95	1.80–1.95
Endotoxin level	< 0.1 EU/µg	< 0.1 EU/µg	< 0.1 EU/µg	< 0.1 EU/µg
Preparation time	100 min/2 preps	150 min/2 preps	180 min/2 preps	20 h/prep
Binding capacity	500 µg	2000 µg	10000 µg	100 mg

Plasmid DNA Kits