-
Consistent cfDNA recovery from 1–10 mL plasma samples
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Efficient purification of fragmented DNA as small as 50 bp
-
No PCR inhibition regardless of your preferred sample volume
The NucleoMag® DNA Plasma kit is designed for the rapid manual and automated isolation of circulating cell-free DNA from human plasma, derived from e.g., EDTA blood draw tubes or Cell-Free DNA BCT®. The NucleoMag® DNA Plasma kit enables the recovery of fragmented DNA ≥ 50 bp with high efficiency due to a reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Elution can be performed with as little as 50–200 μL elution buffer and the eluted DNA is ready to use for subsequent downstream applications like real-time PCR, digital droplet PCR, next generation sequencing, etc. The NucleoMag® DNA Plasma kit content is composed for cfDNA extractions of 2 mL plasma samples, but it can be scaled individually from 1 mL sample volume up to 10 mL.
| Technology |
Magnetic-bead technology |
| Format |
Highly reactive superparamagnetic beads |
| Processing |
Manual or automated
|
| Sample material |
1–10 mL human EDTA/Cell-Free DNA BCT® plasma
|
| Fragment size |
> 50 bp |
| Typical yield |
Depending on sample source, storage, and quality |
| A260/A280 |
1.6–1.9 |
| Elution volume |
50–200 µL |
| Preparation time |
55 min/24 preps (2 mL; excl. lysis)* |
| Binding capacity |
0.3 µg/µL beads |
Ordering Information
| Product |
Preps
|
Specification |
REF |
| NucleoMag® DNA Plasma |
1 x 48
|
NucleoMag® B-Beads, buffers, Liquid Proteinase K |
744550.1 |
| |
4 x 48
|
as above |
744550.4 |
| |
|
|
|
| Material to be supplied by the user |
| E.g. 24-Square-well Block with silicone lid (lysis plate) |
4
|
24-well blocks with 10 mL flat bottom wells |
740679.4 |
E.g. 24-Square-well Block
U-bottom (separation plate) |
24
|
24-well blocks with 10 mL square wells |
740448.24 |
| E.g. Elution Plate U-bottom (elution plate) |
24
|
96-well microplates with 300 µL u-bottom wells |
740486.24 |
| |
|
|
|
| For use with KingFisher®platform |
|
|
|
| KingFisher® 24 Accessory Kit |
1 set
|
24 Deep-well Blocks, 24-Tip Combs, and Plates for 5 x 24 preparations |
744953 |
| KingFisher® Duo Prime Accessory Kit B |
1 set |
24 Deep-well Blocks, 6-Tip Combs, and Plates for 8 x 6 preparations |
744954 |
| |
|
|
|
| Product accessories |
Pack of
|
Specification |
REF |
| NucleoMag® SEP 24 |
1
|
magnetic separator |
744903 |
| NucleoMag® SEP Maxi |
1 |
magnetic separator |
744902 |
| Snap Tubes (50 mL) |
10 |
50 mL tubes |
740822.10 |
| |
50 |
as above |
740822.50 |
| Liquid Proteinase K |
5 mL |
|
740396 |
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