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Premix Ex Taq (Perfect Real Time) - TKRR039A

TKRR039A

Premix Ex Taq (Perfect Real Time) is a 2X RT-PCR kit specifically designed for fast and sensitive real-time PCR via either SYBR Green Real-Time PCR (qPCR) or probe-based qPCR assays. SYBR Green I Dye and probes for probe/5' nuclease based assays are not included in this RT-PCR kit. The Premix Ex Taq (Perfect RealTime) kit consists of Takara's high fidelity and high performance TaKaRa Ex Taq Hot Start polymerase and a real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). Antibody-mediated hot start technology prevents nonspecific amplification due to mispriming and/or formation of primer-dimers during room temperature reaction assembly. Once the Taq antibody-polymerase complex is denatured during the first cycling step, the Premix Ex Taq polymerase can initiate DNA synthesis.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TKRR039A Premix Ex Taq™ DNA Polymerase (Perfect Real Time), 200 Rxns Premix Ex Taq™ DNA Polymerase (Perfect Real Time), 200 Rxns Login for price  

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Features

  • High specificity and increased amplification efficiency for qPCR
  • Sensitivity down to 10 copies for SYBR Green I or probe detection
  • Compatibility with 5' nuclease assays and with SYBR Green I Dye
  • Compatibility with many commonly used Real-Time PCR instruments

Applications

  • Real-Time PCR using probe-based qPCR assays or SYBR Green I Dye

Components

  • Premix Ex Taq Mix (2X conc.)* 5 x 1 mL
  • ROX Reference Dye (50X conc.) 200 µL
  • ROX Reference Dye II (50X conc.) 200 µL
  • *contains TaKaRa Ex Taq HS DNA Polymerase, dNTP mix and Mg2+