PCR Reagents 

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA or PMAxx™ dye and real-time PCR. The kits contain PMA or PMAxx™ dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public health, and/or antibacterial research.

This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions. There is an option to choose either PMA or PMAxx™ for the viability dye (see below for more information about these dyes). The number of samples that can be treated with PMA or PMAxx™ using the kit may vary depending on sample type. See the product protocol under the downloads tab and references for more information.

Kit contents:

• PMA dye OR PMAxx™ dye, 20 mM in water, 100 uL
• 2X Forget-Me-Not™ qPCR Master Mix, 2 x 1 mL (200 reactions)
• ROX reference dye, 1 mL
• hly primer mix, 5 uM, 400 uL