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  5. Nucleic Acid Extraction
  6. RNA Kits
  7. NucleoZOL, 200 mL - MN740404.200

NucleoZOL, 200 mL - MN740404.200

MN740404.200

The universal reagent for RNA isolation

Brand: Macherey-Nagel

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Features

  • No chloroform, no phase separation, easy procedure
  • High RNA yield and purity from any sample material
  • Small and large RNA in one or in separated fractions

Technology

One-phase extraction

Format

Reagent

Sample material

< 1 x 107 cultured cells, bacteria, and yeast, 
< 100 mg human/animal/plant tissue, 
< 0.4 mL (viral) fluids

Fragment size

Small RNA (10–200 nt). Large RNA (> 200 nt)

Typical yield (total RNA)



Typical yield (large RNA)

Liver: 6-8 µg/mg tissue
Kidney, spleen: 3-4 µg/mg tissue
Muscle, brain, lung: 0.5-1.5 µg/mg 
Cultured cells: 4-10 µg/106 cells
Liver: 5-7 µg/mg tissue
Kidney, spleen: 3-4 µg/mg tissue
Muscle, brain, lung: 0.5-1.5 µg/mg 
Cultured cells: 3-8 µg/106 cells

A260/A280

1.8–2.1

Typical RIN (RNA integrity number)

> 9

Elution volume

Flexible

Preparation time

< 1 h