RNA Kits 

Applications

Purification of human or animal viral RNA and DNA from serum, plasma, swab, and tissue homogenates

Features

• Convenient and highly efficient sample lysis by liquid Proteinase K
• Reliable virus detection from fresh or frozen serum / plasma treated with EDTA / citrate
• Highest sensitivity for DNA and RNA viruses e.g., HIV, HPV, H5N1, HCV, HBV, BTV

Specifications

• Technology: Silica membrane technology
• Fragment size: 100 bp–approx. 50 kbp
• Processing: Centrifugation
• Sample material: Cell-free biological fluids, swabs, and tissue homogenates (< 200 μL; < 400 μL with two loading steps)
• Elution volume: 30 μL
• Binding capacity: 25 μg
• Processing time: 50 min/6 preps

Reliable viral RNA and DNA purification with one kit

NucleoSpin® Virus kit is designed for efficient isolation of high quality viral RNA and DNA from serum, plasma, cell-free biological fluids, or tissue homogenates. The kit already contains carrier RNA, Liquid Proteinase K, and all required buffers. A chaotropic salt containing lysis buffer enables the extraction of viral RNA and DNA quickly and efficiently. Difficult to lyse DNA viruses are processed by a buffer optimized Proteinase K digestion. Carrier RNA improves binding and recovery of low concentrated viral nucleic acids. Lysis buffer combined with ethanol creates an appropriate binding condition for nucleic acids to the columns. The following washing steps remove salts, metabolites, and soluble macromolecular cellular components. The nucleic acids are eluted in water and are ready for use for subsequent downstream applications.

Procedure

High sensitive viral BTV RNA detection with NucleoSpin® Virus kit

Viral BTV RNA was isolated from 12 different bovine EDTA blood samples with different virus titer using NucleoSpin® Virus and a competitor kit Q. BTV RNA was quantified by Real-time RT-PCR. The average CT value is lower for NucleoSpin® Virus indicating higher viral RNA yields.

Data kindly provided by Dr. Hoffmann, Friedrich-Loeffler-Institut, Germany

Reliable viral CMV DNA recovery with NucleoSpin® Virus kit

NucleoSpin® Virus and a competitor kit R were used for viral CMV DNA isolation from different plasma samples with diverse virus titer. The viral DNA were quantified by qPCR in Roche LightCycler® 480. The NucleoSpin® Virus shows consistent reliable CMV DNA recovery.

Data kindly provided by Dr. Tiemann, Dipl. Biol. Hartmann, LABCON-OWL GmbH, Germany