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NucleoSpin RNA Plus XS

New generation for efficient and fast RNA isolation from smallest samples

Brand: Macherey-Nagel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MN740990.10 NucleoSpin® RNA Plus XS, 10 preps NucleoSpin® RNA Plus XS, 10 preps Login for price  
MN740990.50 NucleoSpin® RNA Plus XS, 50 preps NucleoSpin® RNA Plus XS, 50 preps Login for price  
MN740990.250 NucleoSpin® RNA Plus XS, 250 preps NucleoSpin® RNA Plus XS, 250 preps Login for price  

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Features

  • Developed for extra small samples down to single cell analysis
  • gDNA removal column to omit time consuming DNA digestion
  • Novel lysis buffer without mercapthoethanol or TCEP

Applications

  • RNA isolation from small amounts of cultured cells, human / animal tissue
  • RNA from samples stored in RNAlater
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays, RNA-sequencing
Product

Preps

Specification REF
NucleoSpin® RNA Plus XS

10

NucleoSpin® RNA Plus XS Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns XS, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers MN740990.10
 

50

as above MN740990.50
 

250

as above MN740990.250
Technology Two column silica-membrane system
1. column for DNA removal
2. column for RNA isolation
Format Mini spin columns – XS design
Sample material

1–105 cultured cells 
< 5 mg human / animal tissue

Fragment size > 100 nt
Typical yield

500-2000 ng (105 HeLa cells)
0.05-0.2 ng (101 HeLa cells)
2.5-8 ng (0.5 µg mouse liver)
0.1-0.5 ng (0.5 µg mouse brain)

A260/A280 1.9–2.2
A260/A230 1.5-2.5
Typical RIN (RNA integrity number) > 8
Elution volume 5–30 µL
Preparation time 18 min/6 preps
Binding capacity 110 µg