Rapid Mini preparation of plasmid DNA in proven 96‑well format
Application |
High copy plasmid DNA purification |
Target |
Plasmid DNA |
CE certified |
No, research use only |
Technology |
Silica membrane technology |
Brand |
NucleoSpin |
Format |
96-well plate |
Package unit |
96 Prep(s), 384 Prep(s), 2304 Prep(s) |
Handling |
Centrifugation, vacuum |
Lysate clarification |
NucleoSpin Plasmid Filter Plate |
Automated use |
Yes |
Application note available with |
Hamilton, Tecan |
Sample material |
Bacteria, E. coli |
Sample amount |
1−5 mL |
Vector size |
< 25 kbp |
Typical yield |
4−30 µg |
Theoretical binding capacity |
20 µg |
Typical purity A260/A280 |
1.8−1.85 |
Endotoxin level |
> 50 EU/µg DNA |
Elution volume |
75−150 µL |
Preparation time |
45 min/plate |
Typical downstream application |
Cloning, PCR, Restriction analysis, Sequencing, Transformation |
Storage temperature |
18−25 °C |
Shelf life |
27 Months |
Hazardous material |
Yes |