Nucleic Acid Extraction 

Isolation of microbial DNA, RNA, or both from diverse water and air samples

• Suitable for diverse salty and freshwater samples, ranging from turbid to clear as well as with air filters
• Minimized inhibition for reliable results
• Compatible with a variety of filters and filtration systems
• Can be additionally combined with two different sizes of ceramic bead tubes for optimal sample lysis

Please note that this kit requires additional accessories that must be used 

•Filters and Filter device - if you don't already have your own, we can supply our PORAFIL cellulose acetate filters.
•Homogenising bead tubes and bead tube holder - can be bought directly from this page
•Plastics and Magnets - check out our Nucleomag Sep device if you require a compatible magnet for separation. 

Filter Compatibility

NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality through determination of the microbial load of the sample.

Microbial nucleic acids from water and air

The new NucleoMag® DNA/RNA Water enables manual or au‑ tomated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environ‑ mental microbiology as well as for estimation of water quality through determination of the microbial load of the sample.

Procedure

Efficient detection for different water and air samples

Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag® DNA/RNA Water kit.

Compatibility with different filtration systems

A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different fil‑ tration systems.