1st generation anion exchanger for purification of plasmid DNA
Application |
High and low copy plasmid DNA purification |
Target |
Plasmid DNA |
CE certified |
No, research use only |
Technology |
Anion exchange chromatography |
Brand |
NucleoBond |
Format |
Giga prep |
Package unit |
5 Prep(s) |
Handling |
Gravity flow |
Lysate clarification |
Folded filter |
Automated use |
No |
Sample material |
Bacteria, E. coli |
Sample amount |
500−2000 mL (high copy), 1−4 L mL (low copy) |
Vector size |
< 300 kbp |
Typical yield |
2000−10000 µg |
Theoretical binding capacity |
10000 µg |
Typical purity A260/A280 |
1.8−1.95 |
Endotoxin level |
1−10 EU/µg DNA |
Isopropanol precipitation |
Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi |
Preparation time |
120−150 min/2 preps |
Typical downstream application |
Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature |
18−25 °C |
Shelf life |
39 Months |
Scope of delivery |
NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A |
Hazardous material |
Yes |