SMARTer universal low input RNA kit

The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA from very low amounts of input RNA that is compromised, has a low RIN value, and/or lacks a poly(A) tail. The kit was specifically designed for RNA-Seq applications involving FFPE or laser-captured samples, and works with as little as 200 pg and up to 10 ng of rRNA-depleted material. It outperforms existing methods in terms of gene-body coverage, data reproducibility, read mappability, and ERCC correlation. For upstream rRNA removal from 10-100 ng of input total RNA, we recommend the RiboGone - Mammalian kit.

Brand: Takara

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
CLT634940 SMARTer® Universal Low Input RNA Kit for Sequencing, 25 Rxns	SMARTer® Universal Low Input RNA Kit for Sequencing, 25 Rxns	Login for price
CLT634938 SMARTer® Universal Low Input RNA Kit for Sequencing, 10 Rxns	SMARTer® Universal Low Input RNA Kit for Sequencing, 10 Rxns	Login for price

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The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis

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