The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.
Required Products
- 639207 Advantage® 2 PCR Kit, 30 Rxns
- 638504 SeqAmp™ DNA Polymerase, 50 Rxns
- 638509 SeqAmp™ DNA Polymerase, 200 Rxns