SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzer sequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.
Required Products
- 639207 Advantage® 2 PCR Kit, 30 Rxns
- 638504 SeqAmp™ DNA Polymerase, 50 Rxns
- 638509 SeqAmp™ DNA Polymerase, 200 Rxns
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