Next Gen Sequencing Kits 

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Overview

• Strand information—Identify each transcript’s strand of origin with >99% accuracy.
• Fast, streamlined protocol—Go from start to finish in ~5 hours.
• Broad input range—Use 100 ng–1 µg of total RNA from human, mouse, or rat.
• Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
• Seamless integration with Illumina sequencing—Generate up to 192 uniquely indexed Illumina-ready libraries.

Applications

• RNA-seq for mammalian samples on Illumina platforms
• NGS library generation that retains strand information
• Analysis of coding and noncoding sequence information