Next Gen Sequencing Kits 

The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.

Overview

• Strand information—Identify each transcript’s strand of origin with >99% accuracy.
• Fast, streamlined protocol—Go from start to finish in ~5 hours.
• Variable input size—Use 10–100 ng of total RNA from human, mouse, or rat.
• Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
• Seamless integration with Illumina sequencing—Generate 24 uniquely indexed Illumina-ready libraries.

Applications

• Robust NGS library construction that retains strand information
• Use for RNA-seq on all Illumina platforms
• Captures coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples