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  6. SMARTer Stranded RNA-Seq Kits

SMARTer Stranded RNA-Seq Kits

The SMARTer Stranded RNA-Seq Kit provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA, and yields robust data from as little as 100 pg to 100 ng of input RNA. The kit's core SMART technology provides complete transcriptome coverage for both coding and noncoding RNA, and the incorporation of Illumina indexes and adapters is included in the workflow, eliminating the need for a downstream library preparation kit. For high-throughput applications, we also offer the SMARTer Stranded RNA-Seq Kit HT, which includes primer sets that allow for multiplexing of up to 96 sequencing libraries on a single flow-cell lane.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT634839 SMARTer® Stranded RNA-Seq Kit, 96 Rxns SMARTer® Stranded RNA-Seq Kit, 96 Rxns Login for price  
CLT634838 SMARTer® Stranded RNA-Seq Kit, 48 Rxns SMARTer® Stranded RNA-Seq Kit, 48 Rxns Login for price  
CLT634837 SMARTer® Stranded RNA-Seq Kit, 24 Rxns SMARTer® Stranded RNA-Seq Kit, 24 Rxns Login for price  
CLT634836 SMARTer® Stranded RNA-Seq Kit, 12 Rxns SMARTer® Stranded RNA-Seq Kit, 12 Rxns Login for price  
CLT634862 SMARTer® Stranded RNA-Seq Kit HT, 96 Rxns SMARTer® Stranded RNA-Seq Kit HT, 96 Rxns Login for price  

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The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

The SMARTer Stranded RNA-Seq Kit HT includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform in a high-throughput manner. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the Indexing Primer Set HT for Illumina (which contains 8 indexed forward PCR primers and 12 indexed reverse PCR primers for the high-throughput amplification of 96 uniquely-indexed RNA-seq libraries). This kit generates indexed, paired-end, Illumina-compatible sequencing libraries in a 96-well plate format, which enables high levels of multiplexing of NGS library analysis.

The SMARTer Stranded RNA-Seq Kit HT utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic cleanup or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. The Indexing Primer Set HT for Illumina integrated into this kit makes it convenient to generate 96 uniquely-indexed libraries for Illumina sequencing.

Overview

  • Accurate—Identify each transcript's strand of origin with >99% accuracy.
  • Sensitive—Detect low-abundance transcripts from as little as 100 pg of input RNA. It is recommended to use rRNA-depleted or poly(A)-purified RNA.
  • Integrated with Illumina sequencing—Incorporate Illumina indexes and adapters during PCR amplification.
  • Couldn't get easier—Go from start to finish in less than 4 hours.
  • SMARTer Stranded RNA-Seq Kit HT—(Cat. # 634862) Uses Illumina high-throughput indexes to generate up to 96 uniquely indexed RNA-seq libraries. All other SMARTer Stranded RNA-Seq Kits utilize 12 low-throughput indexes.

Applications

  • RNA-seq for prokaryotic or mammalian samples on Illumina platforms
  • NGS library generation that retains strand information
  • Analysis of coding and non-coding sequence information