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SMARTer smRNA-Seq Kit for Illumina

Discovery and analysis of small non-coding RNAs (smRNAs) has become an important part of understanding gene expression regulation. Some of the well-known small RNA species are miRNAs, siRNAs, piRNAs, and snoRNAs, many of which range in size from 20 to 30 nt. The SMARTer smRNA-Seq Kit for Illumina has been specifically designed to generate high-quality Illumina-ready sequencing libraries from low-input amounts of small RNAs such as these.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT635029 SMARTer® smRNA-Seq Kit for Illumina®, 12 Rxns SMARTer® smRNA-Seq Kit for Illumina®, 12 Rxns Login for price  
CLT635031 SMARTer® smRNA-Seq Kit for Illumina®, 96 Rxns SMARTer® smRNA-Seq Kit for Illumina®, 96 Rxns Login for price  
CLT635030 SMARTer® smRNA-Seq Kit for Illumina®, 48 Rxns SMARTer® smRNA-Seq Kit for Illumina®, 48 Rxns Login for price  

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The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on Illumina platforms. This kit was developed to work directly with total RNA or enriched small RNA (including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, and primers that include locked nucleic acids (LNAs), this kit allows users to analyze a wide range of smRNA species and generate sequencing libraries of considerable complexity from as little as 1 ng of input material. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.

Overview

  • Analysis of diverse small RNA species—Sequencing library prep of small RNAs ranging in size from 15–150 nt
  • Flexible sample inputs—Start from 1 ng–2 µg of total RNA or enriched small RNA
  • Illumina-ready sequencing libraries—Ligation-free incorporation of Illumina adapters and indexes for sample multiplexing
  • Quick, single-tube workflow—Generate sequencing libraries in ~3 hours (not including validation and post-PCR size-selection steps)

Applications

  • Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15–150 nt