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  5. Next Gen Sequencing Kits
  6. SMARTer Mouse TCR a/b Profiling Kit

SMARTer Mouse TCR a/b Profiling Kit

The SMARTer Mouse TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor (TCR) repertoire analysis using NGS. The kit employs a 5'-RACE-based approach to capture complete V(D)J variable regions of TCR transcripts, starting from as little as 10 ng to 500 ng of total RNA obtained from mouse spleen, thymus, or PBMCs, or from 1,000 to 10,000 purified T cells. As the name suggests, the kit can be used to generate data for both TCR-alpha and TCR-beta chain diversity, either in the same experiment or separately.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT634402 SMARTer® Mouse TCR a/b Profiling Kit, 12 Rxns SMARTer® Mouse TCR a/b Profiling Kit, 12 Rxns Login for price  
CLT634403 SMARTer® Mouse TCR a/b Profiling Kit, 48 Rxns SMARTer® Mouse TCR a/b Profiling Kit, 48 Rxns Login for price  
CLT634404 SMARTer® Mouse TCR a/b Profiling Kit, 96 Rxns SMARTer® Mouse TCR a/b Profiling Kit, 96 Rxns Login for price  

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You are enquiring about SMARTer Mouse TCR a/b Profiling Kit

The SMARTer Mouse TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor (TCR) repertoire analysis using NGS.

Overview

  • Complete V(D)J sequence information—obtain full-length sequences for variable regions of TCR mRNA transcripts.
  • TCR-alpha and TCR-beta—profile diversity for both TCR subunits, either in the same experiment or separately.
  • No multiplex PCR required—amplify sequences for each TCR subunit with a single primer pair per reaction.
  • Illumina-ready sequencing libraries—incorporate Illumina adapter and index sequences in a ligation-independent manner, and multiplex up to 96 libraries in a single flow-cell lane.

Applications

  • Mouse TCR repertoire analysis (TCR-alpha and TCR-beta subunits)