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  5. Next Gen Sequencing Kits
  6. SMARTer Mouse BCR IgG H/K/L Profiling Kit

SMARTer Mouse BCR IgG H/K/L Profiling Kit

The SMARTer Mouse BCR IgG H/K/L Profiling Kit pairs 5' RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling. The 5'-RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT634422 SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 12 Rxns SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 12 Rxns Login for price  
CLT634423 SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 48 Rxns SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 48 Rxns Login for price  
CLT634424 SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 96 Rxns SMARTer® Mouse BCR IgG H/K/L Profiling Kit, 96 Rxns Login for price  

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You are enquiring about SMARTer Mouse BCR IgG H/K/L Profiling Kit

The SMARTer Mouse BCR IgG H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) diversity from total RNA samples and whole cells. This kit is designed to work with a range of RNA inputs, from 10 ng to 3 µg of total RNA obtained from 1,000 to 10,000 purified B cells. This kit can be used to generate data for both heavy (IgG only) and light chain diversity. The kit is not intended to identify the subclasses of IgG heavy chain that are expressed (i.e., IgG1, IgG2a, IgG2b, IgG2c, or IgG3).

This kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of BCR transcripts. Included in the kit are primers that incorporate Illumina-specific adaptor sequences during cDNA amplification.

Overview

  • Input: 10 ng–3 µg of total RNA from spleen, lymph node, PBMCs, and hybridomas
  • Sensitive and specific clonotype detection with optimized cDNA library generation
  • Accurate amplification of mouse IgG subclasses and identification via sequencing in a majority of cases
  • Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
  • Flexible pooling strategies accommodate different experimental requirements regarding alignment and identification of primary chain sequences

Applications

  • Mouse BCR repertoire analysis (heavy and light [kappa and lambda] chains)