RiboGone - Mammalian

The RiboGone - Mammalian kit specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) from human, mouse, or rat total RNA samples. RiboGone - Mammalian uses oligo hybridization and RNase H digestion to specifically deplete 5S, 5.8S, 18S, and 28S nuclear rRNA, as well as 12S mtRNA. The kit is designed to work with input RNA of any quality, including degraded material (e.g., FFPE samples). RiboGone - Mammalian is highly suited for sample preparation prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. When used in tandem with the SMARTer Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit, RiboGone - Mammalian reduces the frequency of rRNA sequences to <5% of total reads.

Brand: Takara

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
CLT634847 RiboGone™ - Mammalian, 24 Rxns	RiboGone™ - Mammalian, 24 Rxns	Login for price
CLT634846 RiboGone™ - Mammalian, 6 Rxns	RiboGone™ - Mammalian, 6 Rxns	Login for price

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RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

Required Products

634836 SMARTer® Stranded RNA-Seq Kit, 12 Rxns
634837 SMARTer® Stranded RNA-Seq Kit, 24 Rxns
634838 SMARTer® Stranded RNA-Seq Kit, 48 Rxns
634839 SMARTer® Stranded RNA-Seq Kit, 96 Rxns
634938 SMARTer® Universal Low Input RNA Kit for Sequencing, 10 Rxns
634940 SMARTer® Universal Low Input RNA Kit for Sequencing, 25 Rxns

Overview

Effective rRNA removal method for human, mouse, and rat total RNA samples—depletes ≥95% of ribosomal RNA sequences
Suitable for any quality RNA, including FFPE samples
Low input working range: 10–100 ng starting material
Works seamlessly with downstream random-primed cDNA synthesis kits like the
Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit

Applications

Removal of rRNA from low-input total RNA samples prior to random-primed cDNA synthesis

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