NEXTFLEX^® PCR-Free Barcodes

• 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences
• Up to 48 multiplexed samples (up to 384 reactions per kit)
• Compatible with NEXTFLEX^® PCR-Free DNA Sequencing Kit
• Considerably reduce your per-sample sequencing cost by barcoded multiplexing
• Increase your sequencing scale by pooling 100s of samples on a single flow cell
• Compatible with Illumina^® Next-Generation Sequencing platforms

Pool Multiple Library Preparations in a Single Flow Cell Lane

NEXTFLEX^® PCR-Free Barcodes are barcoded adapters for multiplexing Illumina^® libraries which provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX^® PCR-Free Barcodes accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads caused by single base errors introduced during PCR. The NEXTFLEX^® index is contained within the adapter sequence, eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end, and multiplex reads and are compatible with the NEXTFLEX^® PCR-Free Sequencing Kits and other PCR-Free DNA library prep protocols.

KIT CONTENTS

• NEXTFLEX^® PCR-Free Barcode Adapter (50 µM)