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  5. Next Gen Sequencing Kits
  6. NEXTFLEX-HT™ Barcodes

NEXTFLEX-HT™ Barcodes

The NEXTFLEX-HT™ Barcodes are single-index adapters for Illumina® platforms that provide unprecedented flexibility and high-throughput capabilities in sequencing applications. Multiplexing with NEXTFLEX-HT™ Barcodes drastically increases scale while reducing costs, by allowing the user to pool multiple libraries in a single flow cell lane. These barcodes can be used with single read or paired-end read sequencing and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, and other Illumina® library preparation workflows that involve the ligation of adapters to adenylated fragments.

Brand: Revvity

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Product CodeProduct Product Description Price (Excl gst) Qty Add
PENOVA-514174 NEXTFLEX-HT BARCODES 1-96 192 RXN NEXTFLEX-HT BARCODES 1-96 192 RXN Login for price  
PENOVA-514175 NEXTFLEX-HT BARCODES 97-192 192 RXN NEXTFLEX-HT BARCODES 97-192 192 RXN Login for price  
PENOVA-514176 NEXTFLEX-HT BARCODES 193-288 192 RXN NEXTFLEX-HT BARCODES 193-288 192 RXN Login for price  
PENOVA-514177 NEXTFLEX-HT BARCODES 289-384 192 RXN NEXTFLEX-HT BARCODES 289-384 192 RXN Login for price  

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NEXTFLEX-HT™ Barcodes

 

  • Single-index adapters for multiplexing up to 384 Illumina libraries
  • Increase your sequencing scale by pooling hundreds of samples on a single flow cell
  • Compatible with both paired-end and single-read Illumina® sequencing
  • Maximum flexibility of suitable pooling combinations for Illumina® platforms

 

Ideal for High-Throughput Capabilities for Sequencing Applications

The NEXTFLEX-HT™ Barcodes are single-index adapters for Illumina® platforms that provide unprecedented flexibility and high-throughput capabilities in sequencing applications. Multiplexing with NEXTFLEX-HT™ Barcodes drastically increases scale while reducing costs, by allowing the user to pool multiple libraries in a single flow cell lane. These barcodes can be used with single read or paired-end read sequencing and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, and other Illumina® library preparation workflows that involve the ligation of adapters to adenylated fragments.

Dual Error Correction Prevents Ambiguity

The NEXTFLEX-HT™ Barcodes utilize a single-index adapter containing a 12 nt unique sequence. The 12 nt indices are designed with a Hamming Distance of at least five throughout the entire series of 2380, permitting dual error correction. Dual error correction enables proper differentiation between samples, by preventing ambiguity from PCR errors or sequencing instrument miscalling. The NEXTFLEX-HT™ index is contained within the adapter sequence, making PCR enrichment optional.

 

Reduce Run-to-Run Sample Carryover & Sample Crosstalk

Even users who don’t routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX-HT™ Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. Plus, each pair of consecutive barcodes are fully color balanced and suitable for two-plex sequencing.

 

KIT CONTENTS

  • NEXTFLEX-HT™ Barcode Adapter (25 µM)
  • NEXTFLEX® Primer Mix (12.5 µM)