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NEXTFLEX® DNA Barcodes are indexed adapters, compatible with Illumina® platforms, that provide flexibility and high-throughput capabilities in sequencing applications. Multiplexing with NEXTFLEX® DNA Barcodes significantly increases scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® DNA Barcodes utilize an indexed adapter containing a 6 nt unique sequence. This allows for proper differentiation between samples by preventing poor reads from single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, reducing or eliminating the need to perform PCR.
Brand: Revvity
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NEXTFLEX® DNA Barcodes are indexed adapters, compatible with Illumina® platforms, that provide flexibility and high-throughput capabilities in sequencing applications. Multiplexing with NEXTFLEX® DNA Barcodes significantly increases scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® DNA Barcodes utilize an indexed adapter containing a 6 nt unique sequence. This allows for proper differentiation between samples by preventing poor reads from single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, reducing or eliminating the need to perform PCR.
These barcodes can be used with single, paired-end, and multiplex reads and are compatible with the NEXTFLEX® Rapid DNA-Seq Kit, NEXTFLEX® Methyl DNA-Seq Kit, the NEXTFLEX® Cell Free DNA-Seq Kit and other genomic DNA library prep protocols compatible with Illumina® sequencing.
Registration failure could occur if the color balance was not maintained between the red and green lasers (used to sequence A/C bases and G/T bases, respectively). Read our blog post, Tech Tips – Barcode Recommendations for Low Level Multiplexing, to learn how to avoid registration failure on an Illumina® sequencer caused by lack sufficient index sequence diversity.
Deep Sequencing of NEXTFLEX® DNA Barcode Generated Sequencing. Libraries generated using the NEXTFLEX® DNA Barcodes have a consistent percentage of usable reads.
Do you want to multiplex more than 48 samples? We also offer the NEXTFLEX-96™ DNA Barcode kits, which contain 96 – 8 nt index barcodes in either a 96-well format (cat # NOVA-514105) or in microfuge tubes (cat # NOVA-514106). The NEXTFLEX-HT™ Barcodes are single index barcodes which allow multiplexing of up to 384 Illumina® libraries.
The NEXTFLEX® adapters contain the full complement of sequencing flow cell binding regions (A, B), which eliminates the need to perform PCR to add the barcode tag.
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