Features
- DNA preparation within 5 minutes
- Loading dye included for subsequent gel electrophoresis
- Designed for tail, ear, blood, and even hair samples
Applications**
- Simple sample preparation in one single tube combined with a fast HotStart PCR
- Direct PCR for PCR analysis directly from unpurified and undiluted samples
** Kits to be used for research purposes only
Principle / Procedure
Many mouse genotyping methods are based on the PCR amplification of genes of interest from tail biopsies or ear punches. The NucleoType Mouse PCR kit is designed for rapid mouse typing experiments and covers these standard sample materials including even blood and animal friendly hair samples. Hair samples can be easily collected causing minimal discomfort to the animal and do not require anesthesia. Thus, the NucleoType Mouse PCR kit enables animal friendly and time saving mouse genotyping.
A simple sample preparation within one tube is combined with a fast PCR. Mouse samples (tail clipping, ear punch, blood, or fur hair) are subjected to a quick lysis at room temperature and Proteinase K inactivation at 98 °C within 5 minutes. For optimal results an aliquot from the lysate is used as template for PCR typing.
The NucleoType HotStart PCR Master Mix (2x) enables a fast PCR (30–90 min) and contains a red dye for direct loading onto agarose gels. In addition the NucleoType Mouse PCR kit offers an alternative time-saving direct PCR protocol allowing PCR directly from unpurified and undiluted samples
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