Description
Osteocalcin is the most abundant non-collagenous protein found in the bone. It is produced by osteoblasts as a 49 amino acid protein, which contains 3 glutamine acid residues (Glu 17, 21, and 24) that are gamma carboxylated in a vitamin K dependent manner. While fully-carboxylated osteocalcin has a high affinity for the extracellular matrix of the bone; decreases in pH, caused by the process of bone resorption, result in decarboxylation of this protein. This generates both partially decarboxylated (undercarboxylated) and fully decarboxylated (uncarboxylated) osteocalcin molecules which are released into the circulation due to decreased affinity for the extracellular matrix.
Fully carboxylated osteocalcin requires high calcium concentrations for proper protein folding; however, under/uncarboxylated osteocalcin has no calcium requirement to maintain its structure within the blood. These under/uncarboxylated forms can bind the receptor GPCR6a and act as hormones promoting insulin sensitivity, glucose tolerance, and testosterone biosynthesis. In patients with diabetes, reduced serum levels of osteocalcin are negatively correlated with obesity and insulin resistance. Furthermore, supporting studies in mice have suggested potential therapeutic applications for osteocalcin in both obesity and insulin resistance.
BioLegend's LEGEND MAX™ Osteocalcin (uncarboxylated) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that recognizes the uncarboxylated form of osteocalcin, allowing for specific study of this hormonal form of the protein. The plate is pre-coated with a monoclonal mouse capture antibody. The detection antibody is a biotinylated monoclonal mouse antibody. This kit is specifically designed for the accurate quantitation of human osteocalcin from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
For more information or documentation, please visit: https://www.biolegend.com/en-us/products/legend-max-human-uncarboxylated-osteocalcin-elisa-kit-with-pre-coated-plate-17119
Kit Contents
- Anti-Human Osteocalcin Precoated 96-well Strip Microplate
- Human Osteocalcin Detection Antibody
- Human Osteocalcin Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
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