Insulin-like growth factor (IGF) is an important hormone for childhood growth and in a variety of physiological functions in adults. Free IGF has a short half-life in serum of about 10 minutes. This half-life is extended by forming a binary complex with IGFBP1-6 to 30 - 90 minutes. IGFALS binds the IGF/IGFBP3 or IGBP5 complex to generate a ternary complex with a serum half-life of more than 12 hours. About 80% of circulating IGF is in this ternary complex.
Insulin-like growth factor binding protein, acid labile subunit (IGFALS), is produced by the liver and is present at high concentrations in postnatal serum. IGFALS is encoded by the IGFALS gene and stimulated by growth hormone. Defects in the IGFALS gene result in lower circulating levels of IGF-1 and IGF-2, causing delayed puberty, increased risk of metastatic disease, and is associated with insulin resistance. Different conditions such as fasting, catabolic disease, or cirrhosis have been shown to reduce serum IGFALS levels in humans. The IGFALS protein is composed of 578 amino acids with 20 leucine-rich repeats.
BioLegend's LEGEND MAX™ Human IGFALS ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human IGFALS capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human IGFALS antibody. This kit is specifically designed for the accurate quantitation of human IGFALS from cell culture supernatant, serum, plasma and other biological fluids. Free or ternary complex IGFALS can be detected. It is analytically validated with ready-to-use reagents.
For more information and documentation, please visit: https://www.biolegend.com/en-us/products/legend-max-human-igfals-elisa-kit-with-pre-coated-plate-16669
Kit Contents
- Anti-Human IGFALS Pre-coated 96-well Strip Microplate
- Human IGFALS Dectection Antibody
- Human IGFALS Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Menu
X
