Target: Name ADAM metallopeptidase domain 33
Alternative Names: A disintegrin and metalloprotease 33 ELISA Kit; A disintegrin and metalloproteinase domain 33 ELISA Kit; ADA33_HUMAN ELISA Kit; ADAM 33 ELISA Kit; ADAM metallopeptidase domain 33 ELISA Kit; ADAM33 ELISA Kit; C20orf153 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit; Disintegrin and reprolysin metalloproteinase family protein ELISA Kit; DJ964F7.1 ELISA Kit; DKFZp434K0521 ELISA Kit; FLJ35308 ELISA Kit; FLJ36751 ELISA Kit; Metalloprotease disintegrin ELISA Kit; MGC149823 ELISA Kit; MGC71889 ELISA Kit; PRO1891 ELISA Kit; UNQ873 ELISA Kit
Abbreviation: ADAM33
Uniprot No.: Q9BZ11
Species: Homo sapiens (Human)
Sample Types: serum, cell culture supernates, saliva, tissue homogenates
Detection Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 7.8 pg/mL
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection Wavelength: 450 nm
Research Area: Cell Biology
Assay Principle: quantitative
Measurement: Sandwich
Precision:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity:
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample |
Serum(n=4) |
|
|
1:1 |
Average % |
97 |
Range % |
92-101 |
1:2 |
Average % |
89 |
Range % |
83-93 |
1:4 |
Average % |
92 |
Range % |
88-96 |
1:8 |
Average % |
95 |
Range % |
90-100 |
Recovery:
The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type: Serum (n=5)
Average % Recovery:93
Range: 89-97
Storage: Store at 2-8°C. Please refer to protocol.