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  5. Pan T Cell Isolation Kit, human

Pan T Cell Isolation Kit, human

MB130-096-535

The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit gives you even higher purity, greater recovery, and faster cell isolation. You can isolate untouched T cells in as little as 18 minutes! For research use only. Size: for 1×10^9 total cells

Brand: Miltenyi Biotec

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Detailed separation procedure
Non-target cells, i.e., monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the Pan T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.
Downstream applications
T cells isolated with the Pan T Cell Isolation Kit can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation. T cells isolated with the Pan T Cell Isolation Kit have also been used to generate antigen-specific T cell lines. Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive or memory T cells and activated T cells.
Columns
LS or autoMACS® Columns.